It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Time-resolved phosphorescence. ©2010 Molecular Devices, Inc. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. Rating: 3. 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. 1 a. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. For more sample throughput, the system canSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. Molecular Devices: n/a:. High base plate design is compatible with automated handling (gripping) and bar coding. Four wash/rinse bottles are included and configurable for both 96- and 384. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Dimensions: 18. (96- and 384-well with pipetting) 6-384. SpectraMax Mini Multi-Mode Microplate Reader (Molecular Devices P/N SMAX MINI AF), with fluorescence filter cube FL-535 (Molecular Devices P/N 5089097). for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. #4TI-0214) UltraVision Plate 96 (Brooks Life Sciences cat. Minimum volume and maximum throughput with 96- or. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. This eliminates the need for standard curves, and Flexibility to read a variety of plate formats. Each kit is sufficient for 3 Calcium 6 assay plates and 3 Calcium 6-QF assay plates. This is equivalent to having 511 built-in filters, 384-well: 0. 340 PC384. Visit the supplier page or submit a quote to learn more. UV and visible absorbanceLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. were pipetted into a 96-well black-walled μclear plate. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Reconstitution of reagents. Microplate readers are commonly used in life science laboratories. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 2. A2000 Full Wavelength Microplate Reader, 6 ~ 384 Well Plate, SHANGHAI PEIOU ANALYSIS INSTRUMENT CO. the Gemini XPS reader. The systems can handle any slide-based microarray study, from small one- or two-fluor applications to multiple-fluor high-throughput projects requiring automated. 100 fmol luciferase per well. (1536-well plate) 2 µL (quartz cuvette), 4 µL (disposable cuvette). The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 96-, 384-, and 1536-well microplates. Note: These settings are based on necessary cookies. x. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. xABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. VIS absorbance detection. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. The default pipettor dispensing speed is 50 µL/sec for 96-well plates and 20 µL/ sec for 384-well plates. FlexIII. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. 375 pM 1 FP Fluorescence Polarization detection for fluorescein- or rhodamine-like labels Using specific LED and EX/EM filters for 6- to 1536-well plates 0200-7009POS 0200-7010POS Fluorescein FP EX 485 nm, EM 535P and 535S nm Rhodamine FP EX 535 nm, EM 595P and 595S nm 96–1. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. It can be performed using any well in the plate with a relatively strong fluorescent signal (i. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The semi-automated PX1 PCR Plate Sealer delivers reliable uniform sealing of a range. x. A Costar 24-well tissue culture plate with col1 = blanks and doubling cell5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12Reader Documentation Version No. ) 0. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. 01% BSA starting at a concentration of 1 x 10 6 fg/well was prepared, and 25 μL of each concentration was added in triplicate to a 384-well solid white plate. Experience the identifiably different. FlexIII. 1. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. 314 low lamp level warning. x. x. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. 1. Plate stacker and robot integration The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. plates < 100 amol (384-well) 1 0200-7018. Molecular Devices products are warranted to meet the stated specifications. Molecular Devices Gemini XS Microplate Spectrofluorometer Details. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Molecular Devices SpectraMax Plus 384 Absorbance Reader Details. Overview. FLIPR Calcium 4 Assay Kit. #3635) Transferpette micropipette (Drummond Scientific cat. Contact Us Phone: +1-800-635-5577 Web: Email: [email protected] nM Z Factor at EC. 1/17 1907B Printed in USA . Formats available are strip washers, full plate washers and combination washer-dispensers. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. All stand-alone instrument functions can be controlled using the software. Microplates, cuvettes (via adapter) Detectors. 2. com Check our website for a current listing of worldwide distributors. That explains the lower values on the LmaxII384 plate reader. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum well volume needed to run a 384 well plate? Minimum volume. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. or use the modified protocol below for the generation of NPCs (see Note 1). 00. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. For more sample throughput, the system can384 Well Plate, supplied by Molecular Devices LLC, used in various techniques. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 384 well polystyrene microplates have plate dimensions of 127. x. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates andThe Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. Programmable 10% to 100%: Sample Cell Options: 96-, 384- or 1536-well microwell plates. Microplate Formats: 6, 12, 24, 48, 96, 384. Pre-read measurements were made with 200 µL water in each well of a Costar UV plate, after which the plate was emptied and blotted dry. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds, Monochromator, tunable 1. Experience the identifiably different. e. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. 1536-well plates < 100 amol (384-well) 1 HTRF • Cisbio HTRF detection with optimized Xenon light source and . #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. 384-Deep Square Well Plates. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as. of samples in both 96- and 384-well microplates or in a cuvette at a selected wavelength for a single point in time (endpoint), over a specified period of time (kinetic), or over a selected wavelength range (spectral scan. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. 384-well TC plate, Poly-d-Lysine, Flat Clear. Therefore, it is recommended that the pipettor height to be set to correspond to the fluid volume in the cell plate prior to compound addition. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Polypropylene deep well plates: Elevated well walls for easier sealing Round. Infinite possibilities. Our microplate. 5 pM 2. 6-384 (96- and 384-well with pipetting) 96-well microplates. [Carbachol] (nM) RFU Max. 1. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. #PLUS 384) Methods. Shaking: Linear, orbital, and double orbital. Can read 96 well plates (optional 384 well). Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. the use of a reader calibrated with primary NIST and NMI standards. x. EMax. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. Plate chamber design of the SpectraMax category 1 microplate readers. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Pfa1 and Pfa1 Gpx4-KO cells stably overexpressing murine FSP1 were seeded on separate 384-well plates (500 cells per well), and screened with a library of small-molecule inhibitor compounds, as. 384-well TC plate, Poly-d-Lysine, Flat. For more sample throughput, the system canThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Supplier: Molecular Devices. The Embla 96/384 Well Washer accepts 96-and 384-well format microplates and is ideal. The Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. CAPABILITIES OF THE EQUIPMENT: The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring. Also refer to this Multimode Reader Comparison. …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. 9. 384-well SBS microplates are designed with corners cut at a1 and h1 positions to conform to SBS nomenclature. UV absorbance detection. com 上海 电话: 86-21-3372 1088 传真: 86-21-3372. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The SpectraMax 190 reader can be integrated with Molecular . x. Dimensions 128 x 86 x 15 mm. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Next-generation luminescence plate reader. Versa Max. x. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. ". Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. flat meniscus), but is approx 15 mL. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. For more sample throughput, the system canPlate Background OD, which was used for subsequent tests, was measured as follows. 306 plate air cal. MultiWash+ Microplate Washer. Some have optional microplate readers, allowing emission scans in up to 384-well plates, for example. Description. Each kit is sufficient. 96- and 384-well. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Selects either 96-well or 384-well plate for a stand-alone read. The former have been the traditional domain of plate readers and great strides have been. #S1207. These robust readers have been placed in labs from Antarctica to the. Select Start>Control panel>Device Manager. Inquiry Basket. This can increase throughput and efficiency, thus saving time and space in the lab. 5577. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. . 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax® Paradigm Plate Reader Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell Molecular Devices, LLC, 1311 Orleans Drive, Sunnyvale, CA 94089 There has been a trend in the past decade from biochemical assays toward cell based assays. Microplate Readers. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. Multichannel processes: Costar 96-well (3300) and Greiner 384-well (781182/781185) microplates are validated for multichannel processes. For more sample throughput, the system canThe SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 384 only) Selects either 96-well or 384-well plate for a stand-alone read. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. easy to use and read multiple plates very quickly. Wash heads are made of Polyethylene Terephthlate (PET) with stainless steel probes to prevent corrosion. Recertification service. tools to expand the boundaries of their research. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. The washer has a small footprint and is vacuum and pressure free, The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. 305 reference level saturation. Full-skirted standard or extra. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. x. 96-deep-round-well-plate, 384-deep-square-well-plate, 24-deep-well-plates. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. minutes and begin reading microplates with seven mouse clicks. Made from polypropylene with conical well profiles to maximize recovery and allow working with sample volumes as low as 5 µL. flat meniscus), but is approx 15 mL. Cell viability는 세포막 Integrity 또는. San Jose, CA 95134. The FilterMax F3 can read 96-well and 384-well plates. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. Catalog number: AB1384W. What are the dimensions of the plate? Code. VMax . 039. Step 1: Open the SoftMax Pro IMAP FP protocol. x. Integrated stacker option. F3/F5 Multi-Mode Microplate ReadersBoth reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. External Dimensions without lid (mm). SpectraMax iD5. 2. #ID5) HTRF Detection System (Molecular Devices cat. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. 96-well fluorescent cell dilution series, top-read. 312 gain calibration failed. After reading the ELISA plate on both readers, each standard curve was nearly identical. Contact; Bioz Stars; Bioz vStars; 86 : Buy from Supplier : Structured Review. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. They are also fully compatible with the FlexStation 3 Multi-Mode Microplate Reader. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. These advertising cookies will no longer be set for this browser and device. 1 fmol luciferase per well. 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. #N207) LAL Reagent Water (Lonza cat. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. 0. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with. Optimizing settings in Standard 384 well black plate A standard black 384 well plate was set up as described in above Table 4. 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. 190. More Product Information. 384 wells, the SpectraMax iD3 reader. • Tyrosine Kinase Plate Please contact your Molecular Devices sales representative. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. 100 fmol 1536-well (8 µL) 0. Page 154: Ordering Parts And Accessories. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Wash heads are made of Polyethylene Terephthlate (PET) with stainless steel probes to prevent corrosion. Molecular Devices deliver high throughput while showing good correlation with manual patch clamping data. Cells toxicity was corrected for the culture medium background absorbance. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. It is also important to note that organoid growth patterns are relatively consistent from 24- to 384-well plates. Embla washers are durable and easy to maintain. Multimode Microplate Reader. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. x. 2 with 1000 cells. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Round well design with a clear flat bottom 2. Microplate format. The calculated LOQ values were 0. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. If no reference reading is taken, the instrument uses the I 0 values stored in the NVRAM (non-volatile memory) of the. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. Total volume (except deep. Browse for different fluorescent readers below and compare across different instrument suppliers. 48 ± 0. explore more. Published Dec 16, 2020 | Updated Dec 16, 2020. Dispense 10 µL of each ATP/ADP combination across an entire row of a 384-well plate. Microarray scanners for one-fluor to multi-fluor [email protected] and Download Molecular Devices SPECTRAmax PLUS 384 operator's manual online. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Figure 3. The Embla 96/384 Well Washer accepts 96-and 384-well format microplates and is ideal. B. SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. 2 with 1000 cells. For biochemical assays, the washer removes unbound material and unreacted reagents. Filter. No accessories are needed and SoftMax® Pro Software. x ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. Plate reader instructions Computer. Fluid dispensing speed. Summary of Contents for Molecular Devices SpectraMax M2. Expanded wavelength coverageIn the software select Tools > Configuration. ‘StimB’ is Stimulation Buffer, included in the IP-One Gq kit. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). The FLEXstation II 384 (Molecular Devices, Inc. Ideal for miniaturized 384 well fluorescent. Fluorescent Imaging Plate Reader (FLIPRTM ) technology to measure Ca2+ flux in cells was an important development in the early 1990’s and has played a significant role in HTS and lead optimization applications. 1. Ensure your assay quality with quality tips Certified RNase-/DNase-free Nonpyrogenic Nonsterile 50 racks/case. Includes PC and software. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. Inquiry Basket. High base plate design is compatible with automation and for bar coding. 50 μL total well volume, with recommended working volume of 5-40 μL. 384 well plates 6–384 wells 6–384 wells 6–384 wells 6–1,536 wells (fluorometry, TRF, luminometry, AlphaScreen assays). Another consideration, says Amouretti, is plate format. Temperature control: 4°C above ambient to 45°C. Pick the best speed, sensitivity, and price for your needs. Was the failure only of the Detection Estimate (Mag3) Test for the M3/M4/M5/M5e/Flex3?Gurard-Levin describes his lab’s microplate reader selection: “In our labs, specific and refined optics are important, as well as the ability to use high density plates including those with 384- or 1536-wells. SpectraMax i3x. Turn the. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. Loading Buffer was also added to a set of cell-free wells for background controls. MultiWash+ Microplate Washer. VIS absorbance detection. X. M5. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. 7°C (gradient heating design). Suggested plates are black. Efficiency. 2 OD at 200 nm; this decreased to 0. The plate reader consisted of a large-area illumination unit and a parallel detection unit; see Fig. SpectraMax ID5 Plate Reader (Molecular Devices). Using Molecular Devices microplate readers and SoftMax. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. x. x. All stand-alone instrument functions can be controlled using the software. Technical specifications. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. 20 attomol ATP per well. Selects either 96-well or 384-well plate for a stand-alone read. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofThe plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. • The FlexStation™ II instrument is designed specifically for 96-well microplates. Uncontrolled Copy for Training and Reference Purposes Only . Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. These lab workhorses are used in drug discovery, research, bioassay validation, and. Fluorescence Microplate Reader Comparison Chart. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. 9. 96-, 384-, and 1536-well microplates. Danaher Inc 384 well plate 384 Well Plate, supplied by Danaher Inc, used in various techniques. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax Plus 384 Microplate Reader (Molecular Devices cat. Temperature Control: ambient from +4 to 45C. 6-384 (96- and 384-well with pipetting) 96-well microplates. Temperature control. What is the dead volume in 96 and 384 well compound plates? Dead volume is 5 micro liters. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. This chapter is an introductory overview of the most commonly used assay methods to estimate the number of viable cells in multi-well plates. x. For more sample throughput, both readers can be easily. readers are the industry’s most cited instruments and have empowered life science researchers to advance protein and cell biology, breaking the barriers to novel, landmark discoveries. Option 1: Use a plate. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. 0. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 4 Equipment. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today. Two high-energy xenon flash lamps: Reading capabilities. The user-friendly MultiWash™+ Microplate Washer from Molecular Devices, one of the pioneers in microplate detection, includes 20 preset protocols, works with both 96- and 384-well plates, and comes with 4 different wash/rinse bottles for out-of-the-box use. (40 µl maximum) Clear, white, or black. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. (blue) and DsRed (red) transfected cells in 384-well plates, read from the bottom.